beta actin Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86718A
Western Blotting of cell/tissue lysates with anti-Beta Actin monoclonal antibody at dilution of 1:2000.
Lane1: PANC-1 cell lysates, Lane2: 293F cell lysates, Lane3: Human placenta lysates, Lane4: Mouse SP2/0 celll ysates, Lane5: Mouse kidney lysates.
Western blotting of Zebrafish lysates with anti-Beta Actin monoclonal antibody at dilution of 1:2000.
Immunofluorescence analysis of 293 cells with anti-beta actin monoclonal antibody at 1:100 dilution.
Product Name
beta actin Monoclonal Antibody
Catalog Number
RD86718A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG2a
Host
Mouse
Background
Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells, and commonly used as a loading control for western blot and as a control for qPCR.
Immunogen Information
Immunogen
Recombinant human Beta actin protein
Gene ID
57935
Swissprot
Q7ZVF9
Synonyms
ACTBActin betaActxActin cytoplasmic 1b actinA26C1A
Calculated MW
55kDa
Observed MW
55kDa
Applications
Reactivity
Human,Mouse,Rat,Zebrafish
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IF 1:100-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.