beta Tubulin Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86504A
Western Blot analysis of A549, Rat brain, Mouse brain, Chicken lung, Rabbit testis, Sheep muscle using beta Tubulin Monoclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded Mouse testis tissue using beta Tubulin Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Hela tissue using beta Tubulin Monoclonal Antibody at dilution of 1:100.
Product Name
beta Tubulin Monoclonal Antibody
Catalog Number
RD86504A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
Immunogen Information
Immunogen
Synthetic Peptide
Swissprot
Q13509
Synonyms
beta 3 tubulinbeta-4CDCBMCDCBM1CFEOM3CFEOM3AFEOM3M(beta)3M(beta)6MC1RNeuron specific beta III TubulinNeuron-specific class III beta-tubulinQccE-11995QccE-15186TBB3Tubb 3TUBB3TUBB4Tubulin beta 3Tubulin beta 3 chainTubulin beta 4Tubulin
Calculated MW
50 kDa
Observed MW
55 kDa
Applications
Reactivity
Human,Mouse,Rat,Monkey,Chicken,Dog,Hamster,Rabbit,Sheep,Insect,Yeast
Tested Applications
WB,IHC-p,IF
Conjugation
Unconjugated
Dilution
WB 1:5000-1:10000, IHC 1:100-1:300, IF 1:100-1:300
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas