beta Tubulin Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86719A
Western blotting with anti-Beta Tubulin monoclonal antibody at dilution of 1:5000.
Lane 1: Hela cell lysates, Lane 2: Caco-2 cell lysates, Lane 3: HT-29 cell lysates, Lane 4: NIH/3T3 cell lysates, Lane 5:Raw264.7 cell lysates, Lane 6: Rat liver
Immunofluorescent analysis of HEK293 cells with anti-beta Tubulin monoclonal antibody at dilution of 1:4000.
Product Name
beta Tubulin Monoclonal Antibody
Catalog Number
RD86719A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG2a
Host
Mouse
Background
In eukaryotes there are six members of the tubulin superfamily. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis. Both α and β tubulins have a mass of around 50 kDa. Tubulin was long thought to be specific to eukaryotes. Beta-tubulin gene is commonly used as a loading control for western blot.
Immunogen Information
Immunogen
Recombinant human TUBB protein
Gene ID
22154
Swissprot
P99024
Synonyms
Beta 4 tubulinBeta 5 tubulinbeta Ib tubulinBeta1 tubulinClass I beta tubulinTUBB 1TUBB 2TUBB 5TUBBTUBB1TUBB2TUBB5tubulin beta 1 chainTubulin beta 2 chaintubulin beta 5 chainTubulin beta chainTubulin beta class Itubulin be
Calculated MW
40kDa
Observed MW
40kDa
Applications
Reactivity
Human, Mouse, E.coli, Scallop, Zebrafish
Tested Applications
WB, IF
Conjugation
Unconjugated
Dilution
WB 1:5000-1:10000, IF 1:2000-1:4000
Concentration
3mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.