BOP1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89243A
Western blot analysis of extracts of various cell lines using BOP1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat lung using BOP1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse brain using BOP1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Variation
Quantity
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Overview
Product Name
BOP1 Polyclonal Antibody
Catalog Number
RD89243A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
Immunogen Information
Immunogen
Recombinant fusion protein of human BOP1
Gene ID
23246
Swissprot
Q14137
Calculated MW
1011kDa
Observed MW
131KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.