BPGM Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD86876A
Western blot analysis of extracts of various cell lines using BPGM Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using BPGM Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of HeLa cells using BPGM Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using BPGM Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
BPGM Polyclonal Antibody
Catalog Number
RD86876A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Immunogen Information

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Immunogen
Recombinant fusion protein of human BPGM
Gene ID
669
Swissprot
P07738
Synonyms
BPGMDPGM
Calculated MW
76kDa/78kDa
Observed MW
120kDa

Applications

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Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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