BRAF Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD78439A
Western Blot analysis of Hela and Raji cell using BRAF Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Hela and Raji cell using BRAF Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Human cervical cancer using BRAF Polyclonal Antibody at dilution of 1:30
Immunohistochemistry of paraffin-embedded Human cervical cancer using BRAF Polyclonal Antibody at dilution of 1:30
Immunohistochemistry of paraffin-embedded Human prostate cancer using BRAF Polyclonal Antibody at dilution of 1:30
Immunohistochemistry of paraffin-embedded Human prostate cancer using BRAF Polyclonal Antibody at dilution of 1:30
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Overview

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Product Name
BRAF Polyclonal Antibody
Catalog Number
RD78439A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.

Immunogen Information

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Immunogen
Synthetic peptide of human BRAF
Swissprot
P15056
Synonyms
FLJ9510994 kDa B raf proteinB raf 1B Raf proto oncogene serine threonine protein kinaseB Raf proto oncogeneserine/threonine kinaseB RAF1B-Raf proto-oncogene serine/threonine-protein kinase (p94)BRAF 1BRAFBRAFBRAF1cRmilMGC126806MGC138284Muri
Calculated MW
84 kDa
Gene Accession
NP_004324

Applications

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Reactivity
Human,Mouse
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:50-1:200
Concentration
0.7 mg/mL
Storage Buffer
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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