Western blot analysis of extracts of various cell lines using BRAP Polyclonal Antibody at 1:3000 dilution.
Immunohistochemistry of paraffin-embedded human lung cancer using BRAP Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver cancer using BRAP Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm.
Immunogen Information
Immunogen
Recombinant fusion protein of human BRAP
Gene ID
8315
Swissprot
Q7Z569
Synonyms
BRAPBRAP2IMPRNF52
Calculated MW
18kDa/96kDa/110kDa/113kDa/114kDa
Observed MW
114kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.