Brdu Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD70449A
Immunohistochemistry analysis of paraffin-embedded mouse jejunum(intraperitoneal injection BrdU every 2 hours for 4 times) using Brdu Monoclonal Antibody at dilution of 1:300.
Immunofluorescence analysis of Hela cells（treated with 0.03mg/ml BrdU for 40 min ) using Brdu Monoclonal Antibody at dilution of 1:100.
Product Name
Brdu Monoclonal Antibody
Catalog Number
RD70449A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Mouse
Background
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it.
Immunogen Information
Immunogen
KLH conjugated BrdU
Synonyms
Bromodeoxyuridine;Bromodeoxyuridine;5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
IHC,IF
Conjugation
Unconjugated
Dilution
IHC 1:100-1:500, IF 1:100-1:500
Concentration
0.7 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.