BRK1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD267071A
Immunohistochemistry of paraffin-embedded Human breast cancer using BRK1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human stomach using BRK1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using BRK1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
BRK1 Polyclonal Antibody
Catalog Number
RD267071A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
HSPC300 (hematopoietic stem cell protein 300) is also known as probable protein BRICK1 or C3orf10 (chromosome 3 open reading frame 10) and is a 75 amino acid protein that is expressed as two isoforms and localizes to both the cytoplasm and the cytoskeleton. HSPC300 is thought to regulate cytoskeletal organization and Actin polymerization. Free HSPC300 exists as homotrimers prior to its incorporation into the WAVE complex. The WAVE complex includes five proteins, one of which is HSPC300, that regulate the ARC (Arp2/3 complex) which is responsible for Actin nucleation and is Rac 1-dependent. Because HSPC300 is a highly conserved subunit of the WAVE complex across many species, it is thought to have the same or similar functions in many different organisms.
Immunogen Information
Immunogen
Recombinant fusion protein of human BRK1 (NP_060932.2).
Gene ID
55845
Swissprot
Q8WUW1
Synonyms
BRK1C3orf10HSPC300MDS027hHBrk1
Applications
Reactivity
Human,Mouse
Tested Applications
IHC,IF
Conjugation
Unconjugated
Dilution
IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas