c-Fos Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85587A
Western Blot analysis of 1) Hela, 2) 293T, 3) Mouse brain, 4) Rat brain using c-Fos Monoclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using c-Fos Monoclonal Antibody at dilution of 1:200.
Product Name
c-Fos Monoclonal Antibody
Catalog Number
RD85587A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
Immunogen Information
Immunogen
Recombinant Protein
Swissprot
P01100
Synonyms
Activator protein 1AP 1C FOSCellular oncogene c fosCellular oncogene fosFBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS)FBJ murine osteosarcoma viral oncogene homologFBJ murine osteosarcoma viral v fos oncogene homologFBJ Oste
Observed MW
62 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300 ELISA 1:40000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas