c-Fos Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD266884A
Western blot analysis of extracts of various cell lines using c-Fos Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of C6 cells using c-Fos Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using c-Fos Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
c-Fos Polyclonal Antibody
Catalog Number
RD266884A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Immunogen Information
Immunogen
Recombinant protein of human c-Fos
Gene ID
2353
Swissprot
P01100
Synonyms
FOSAP-1C-FOSp55c-Fos
Calculated MW
28 kDa/36 kDa/40 kDa
Observed MW
60 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas