C5AR1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot analysis of extracts of various cell lines using C5AR1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using C5AR1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human appendix using C5AR1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Name
C5AR1 Polyclonal Antibody
Catalog Number
RD89177A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.
Immunogen Information
Immunogen
Recombinant fusion protein of human C5AR1
Gene ID
728
Swissprot
P21730
Synonyms
C5AR1C5AC5ARC5R1CD88
Calculated MW
138kDa/240kDa/249kDa/250kDa/254kDa
Observed MW
254kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas