For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90176A
Immunohistochemistry of paraffin-embedded mouse spinal cord using CALCA Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human CALCA
Gene ID
796
Swissprot
P06881
Synonyms
CALCACALC1CGRPCGRP-ICGRP1CTKCPCT
Calculated MW
10kDa/29kDa/35kDa/42kDa/45kDa
Observed MW
48KDa/20-25KDa
Applications
Reactivity
Mouse
Tested Applications
IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.