Casein Kinase 2 alpha Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89386A
Western blot analysis of extracts of various cell lines using Casein Kinase 2 alpha Polyclonal Antibody at1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat liver using Casein Kinase 2 alpha Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human gastric cancer using Casein Kinase 2 alpha Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using Casein Kinase 2 alpha Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Name
Casein Kinase 2 alpha Polyclonal Antibody
Catalog Number
RD89386A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human Casein Kinase 2 alpha
Gene ID
1457
Swissprot
P68400
Synonyms
CSNK2A1CK2A1CKIICSNK2A3OCNDSCka1Cka2casein kinase 2 alpha 1
Calculated MW
11kDa
Observed MW
Refer to figures
Applications
Reactivity
Human
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas