CASP1 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of Mouse testis using CASP1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Mouse testis using CASP1 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of C6 cells using CASP1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using CASP1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CASP1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CASP1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
CASP1 Polyclonal Antibody
Catalog Number
RD90266A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B and interleukin 18 (IL18 as well as the pyroptosis inducer Gasdermin-D (GSDMD, into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a proinflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT part. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (By similarity.

Immunogen Information

Circle
Immunogen
A synthetic peptide of mouse CASP1
Gene ID
12362
Swissprot
P29452
Synonyms
ICICEIl1CaspIl1bc
Calculated MW
58kDa
Observed MW
85KDa

Applications

Circle
Reactivity
Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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