CASP9 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85497A
Western Blot analysis of Hela cells using CASP9 Monoclonal Antibody at dilution of 1) 1:2000 2) 1:5000.
Product Name
CASP9 Monoclonal Antibody
Catalog Number
RD85497A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Immunogen Information
Immunogen
Synthetic Peptide
Swissprot
P55211
Synonyms
APAF-3APAF3Apoptosis related cysteine peptidaseApoptotic protease Mch-6Apoptotic protease-activating factor 3CASP-9CASP9CASP9Caspase 9 apoptosis related cysteine peptidaseCaspase 9 Dominant NegativeCaspase 9cCaspase-9Caspase-9 subunit p10ICE
Calculated MW
46 kDa
Observed MW
47-51 kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-p,IF,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:50-300, IF 1:50-1:200, IP 1:100-1:300
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas