CASP9 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD70881A
Western Blot analysis of various cells using CASP9 Polyclonal Antibody at dilution of 1:500.
Product Name
CASP9 Polyclonal Antibody
Catalog Number
RD70881A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Immunogen Information
Immunogen
Synthesized peptide derived from human Caspase-9 around the non-phosphorylation site of Thr125.
Swissprot
P55211
Synonyms
APAF-3APAF3Apoptosis related cysteine peptidaseApoptotic protease Mch-6Apoptotic protease-activating factor 3CASP-9CASP9CASP9Caspase 9 apoptosis related cysteine peptidaseCaspase 9 Dominant NegativeCaspase 9cCaspase-9Caspase-9 subunit p10ICE
Calculated MW
46 kDa
Observed MW
46 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas