Caspase-9 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of various cell lines using Caspase-9 Polyclonal Antibody at 1:1000 dilution.L929 cells were treated by staurosporine(1 uM) for 3 hour.
Western blot analysis of extracts of various cell lines using Caspase-9 Polyclonal Antibody at 1:1000 dilution.L929 cells were treated by staurosporine(1 uM) for 3 hour.
Western blot analysis of extracts of various cell lines using Caspase-9 Polyclonal Antibody at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Western blot analysis of extracts of various cell lines using Caspase-9 Polyclonal Antibody at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
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Overview

Circle
Product Name
Caspase-9 Polyclonal Antibody
Catalog Number
RD86753A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Immunogen Information

Circle
Immunogen
A synthetic peptide of human Caspase-9
Gene ID
842
Swissprot
P55211
Synonyms
CASP9APAF-3APAF3ICE-LAP6MCH6PPP1R56caspase-9casp9Caspase 9
Calculated MW
48kDa/52kDa
Observed MW
60KDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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