Catenin beta Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85531A
Western Blot analysis of 1) Hela, 2) 293T, 3) Mouse liver, 4) Rat liver with Catenin beta Monoclonal Antibody
Immunohistochemistry of paraffin-embedded Mouse kidney tissue using Catenin beta Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Human stomach cancer tissue using Catenin beta Monoclonal Antibody at dilution of 1:200.
Product Name
Catenin beta Monoclonal Antibody
Catalog Number
RD85531A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
Immunogen Information
Immunogen
Recombinant Protein
Swissprot
P35222
Synonyms
Beta cateninBeta-cateninCadherin associated proteinCatenin (cadherin associated protein)beta 188kDaCatenin beta 1Catenin beta-1CATNBCHBCATCTNB1CTNNBCTNNB1DKFZp686D02253FLJ25606FLJ37923OTTHUMP00000162082OTTHUMP00000165222OTTHUMP0000016522
Observed MW
92 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.