Caveolin-2 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of various cell lines using Caveolin-2 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using Caveolin-2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat brain using Caveolin-2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat brain using Caveolin-2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat lung using Caveolin-2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat lung using Caveolin-2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using Caveolin-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Caveolin-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
Caveolin-2 Polyclonal Antibody
Catalog Number
RD89325A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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The protein encoded by this gene is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. This gene and related family member (CAV1) are located next to each other on chromosome 7, and express colocalizing proteins that form a stable hetero-oligomeric complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Additional isoforms resulting from the use of alternate in-frame translation initiation codons have also been described, and shown to have preferential localization in the cell (PMID:11238462). [provided by RefSeq, May 2011]

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human CAV2
Gene ID
858
Swissprot
P51636
Synonyms
CAV
Calculated MW
21kDa
Observed MW
30KDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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