Western blot analysis of extracts of THP-1 cells using CCL3 Polyclonal Antibody at 1:1000 dilution.THP-1 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation.
Immunofluorescence analysis of C6 cells using CCL3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using CCL3 Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using CCL3 Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.
Immunogen Information
Immunogen
Recombinant fusion protein of human CCL3
Gene ID
6348
Swissprot
P10147
Synonyms
CCL3G0S19-1LD78ALPHAMIP-1-alphaMIP1ASCYA3
Calculated MW
156kDa/160kDa/162kDa
Observed MW
162kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.