CD22 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90674A
Western blot with CD22 Polyclonal antibody at dilution of 1:500.lane 1:Raji whole cell lysate
Immunohistochemistry of paraffin-embedded Human tonsil tissue using CD22 Polyclonal Antibody at dilution of 1:350
Product Name
CD22 Polyclonal Antibody
Catalog Number
RD90674A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
CD22,also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule),is a 130-140 kDa,B-cell restricted,type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling,preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.
Immunogen Information
Immunogen
Recombinant Human CD22 protein expressed by E.coli
Swissprot
P20273
Synonyms
B cell receptor CD22 precursorB lymphocyte cell adhesion moleculeBL CAMCD 22CD22 antigenCD22 moleculeLectin 2Leu14Lyb8MGC130020sialic acid binding Ig like lectin 2SIGLEC 2T cell surface antigen Leu 14
Calculated MW
34 kDa
Observed MW
45 kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:200-1:400
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas