CD45 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD70568A
Western Blot analysis of various samples using CD45 Polyclonal Antibody at dilution of 1:1000.
Product Name
CD45 Polyclonal Antibody
Catalog Number
RD70568A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
CD45,also known as leukocyte common antigen (LCA),B220,and T200,is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells,except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth,differentiation,cell cycle,and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29,CD44,CD90,CD105 and CD166),negative selection markers (such as CD34 and CD45) are used for MSC identification.
Immunogen Information
Immunogen
Recombinant protein corresponding to Mouse Human CD45.
Swissprot
P08575,P06800
Synonyms
B220CD 45CD45CD45 antigenCD45RGP180L-CALCALeukocyte common antigenlocLy-5LY5Ly5homolog ofLyt-4OTTHUMP00000033813OTTHUMP00000033816OTTHUMP00000033817OTTHUMP00000038574Protein tyrosine phosphatase receptor type c polypeptideProtein tyros
Calculated MW
146 kDa
Observed MW
180-240 kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1.15 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.