CDC25B Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD87111A
Western blot analysis of extracts of A-549 cells using CDC25B Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of A-549 cells using CDC25B Polyclonal Antibody at 1:1000 dilution.
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Overview

Circle
Product Name
CDC25B Polyclonal Antibody
Catalog Number
RD87111A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human CDC25B
Gene ID
994
Swissprot
P30305
Synonyms
CDC25B
Calculated MW
101kDa/102kDa
Observed MW
110kDa

Applications

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Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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