CDK1 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD79983A
Western blot analysis of extracts of various cell lines using CDK1 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using CDK1 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat brain using CDK1 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat brain using CDK1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse spinal cord using CDK1 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse spinal cord using CDK1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using CDK1 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using CDK1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using CDK1 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using CDK1 Polyclonal Antibody
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Overview

Circle
Product Name
CDK1 Polyclonal Antibody
Catalog Number
RD79983A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

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Immunogen
A synthetic peptide of human CDK1 (NP_001777.1).
Gene ID
983
Swissprot
P06493
Synonyms
CDK1CDC2CDC28AP34CDC2
Calculated MW
27 kDa/34 kDa
Observed MW
34 kDa

Applications

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Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:100 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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