CDK1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90335A
Western blot analysis of extracts of various cell lines using CDK1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Jurkat cells using CDK1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using CDK1Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of C6 cells using CDK1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using CDK1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600ug extracts of Jurkat cells using 3ug CDK1 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using CDK1 Polyclonal Antibody at a dilution of 1:1000.
Immunoprecipitation analysis of 600ug extracts of C6 cells using 3ug CDK1 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using CDK1 Polyclonal Antibody at a dilution of 1:1000.
Product Name
CDK1 Polyclonal Antibody
Catalog Number
RD90335A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
A synthetic peptide of human CDK1
Gene ID
983
Swissprot
P06493
Synonyms
CDK1CDC2CDC28AP34CDC2cyclin-dependent kinase 1
Calculated MW
44kDa/69kDa/77kDa/134kDa
Observed MW
175KDa
Applications
Reactivity
Human
Tested Applications
WB,IF,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.