CDK12 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88038A
Western blot analysis of extracts of LNCaP cells using CDK12 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Mouse brain using CDK12 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human breast cancer using CDK12 Polyclonal Antibody at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human esophagus using CDK12 Polyclonal Antibody at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Variation
Quantity
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Overview
Product Name
CDK12 Polyclonal Antibody
Catalog Number
RD88038A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD of the large subunit of RNA polymerase II (POLR2A, thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.
Immunogen Information
Immunogen
Recombinant fusion protein of human CDK12
Gene ID
51755
Swissprot
Q9NYV4
Synonyms
CDK12CRK7CRKRCRKRS
Calculated MW
41kDa
Observed MW
53kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas