CEP55 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD267965A
Western blot analysis of extracts of THP-1 cells using CEP55 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of L929 cells using CEP55 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
CEP55 Polyclonal Antibody
Catalog Number
RD267965A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Centrosomal protein of 55 kDais aproteinthat in humans is encoded by theCEP55gene. CEP55 is a mitotic phosphoprotein that plays a key role in cytokinesis, the final stage of cell division. Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1. Interacts with AKAP9; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PDCD6IP; the interaction is direct; CEP55 binds PDCD6IP in a 2:1 stoechiometry; PDCD6IP competes with TSG101 for the same binding site.
Immunogen Information
Immunogen
Recombinant fusion protein of human CEP55 (NP_060601.3).
Gene ID
55165
Swissprot
Q53EZ4
Synonyms
CEP55C10orf3CT111URCC6
Calculated MW
46 kDa/54 kDa
Observed MW
60 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas