CHAMP1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD219634A
Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using CHAMP1 Polyclonal Antibody at dilution of 1:40(×200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CHAMP1 Polyclonal Antibody at dilution of 1:40(×200)
Product Name
CHAMP1 Polyclonal Antibody
Catalog Number
RD219634A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
CHAMP1 (chromosome alignment maintaining phosphoprotein 1), also known as ZNF828 or C13orf8, is a 812 amino acid protein containing one C2H2-type zinc finger. CHAMP1 is required for the proper alignment of chromosomes during metaphase, undergoing CDK1-dependent phosphorylation at multiple sites during mitosis. The phosphorylation counteracts the negative chromosomal alignment regulation of the zinc-finger domain of CHAMP1. One region of CHAMP1, the FPE region, is responsible for spindle and kinetochore localization, which is essential for proper chromosome alignment. CHAMP1 interacts with MAD2L2, PGOZ, CBX1, CBX3 and CBX5, and may recruit CENPE and CENPF to the kinetochore. The CHAMP1 gene is located on chromosome 13 and is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat and chicken.
Immunogen Information
Immunogen
Synthetic peptide of human CHAMP1
Swissprot
Q96JM3
Synonyms
C13orf8CAMPCHAMPCHAMP1CHAP1chromosome 13 open reading frame 8Chromosome alignment-maintaining phosphoprotein 1FLJ90413KIAA1802Zinc finger protein 828Zinc finger protein KIAA1802ZNF828
Gene Accession
NP115812
Applications
Reactivity
Human, Mouse
Tested Applications
IHC,ELISA
Conjugation
Unconjugated
Dilution
IHC 1:30-1:150, ELISA 1:5000-1:10000
Concentration
0.6 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas