CK-19 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD70293A
Western Blot analysis of various samples using CK-19 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded human liver using CK-19 Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using CK-19 Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded rat kidney using CK-19 Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded Human SmallIntestine using CK-19 Polyclonal Antibody.
Variation
Quantity
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Overview
Product Name
CK-19 Polyclonal Antibody
Catalog Number
RD70293A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19,one of type I keratins,is specifically expressed in the periderm,the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity,KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes,peripheral blood,and bone marrow of breast cancer patients. This antibody,generated against full length KRT19 protein,also recognizes KRT17,another type I keratin homologous to KRT19. KRT17 is used as a marker for trauma.
Immunogen Information
Immunogen
KLH conjugated Synthetic peptide corresponding to Mouse Cytokeratin 19
Swissprot
P08727,P19001,Q63279
Synonyms
40 kDa keratin intermediate filamentCK 19CK-19CK19Cytokeratin 19Cytokeratin-19K19K1C19K1CSKeratin 19Keratin type I 40 kDKeratin type I 40kDKeratin type I cytoskeletal 19Keratintype I cytoskeletal 19Keratintype I40 kdKeratin-19KRT19MGC1
Calculated MW
44 kDa
Observed MW
44 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:200-1:800
Concentration
0.66 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas