CK-8 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD70437A
Western Blot analysis of various samples using CK-8 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded human colon using CK-8 Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry analysis of paraffin-embedded human stomach cancer using CK-8 Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry analysis of paraffin-embedded mouse lung using CK-8 Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry analysis of paraffin-embedded rat stomach using CK-8 Polyclonal Antibody at dilution of 1:300.
Variation
Quantity
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Overview
Product Name
CK-8 Polyclonal Antibody
Catalog Number
RD70437A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Cytokeratin 8 (CK8) has a molecular weight of 52.5 KDa, which belongs to the HMW (high molecular weight) B-type Cytokeratin. CK8 is mainly marked on non-squamous epithelium, thus can be used for the diagnosis of adenocarcinoma and ductal carcinoma. Generally, CK8 is not expressed in squamous cell carcinoma. It is reported that hepatocellular carcinoma mainly express CK8 and CK18.
Immunogen Information
Immunogen
KLH conjugated Synthetic peptide corresponding to Mouse Cytokeratin 8
Swissprot
P05787,P11679,Q10758
Synonyms
CARD2CK 8CK-8CK8CYK8CYKERCytokeratin endo ACytokeratin-8DreK8EndoAK2C8K2C8K8Keratin 8Keratin type II cytoskeletal 8Keratintype II cytoskeletal 8Keratin-8KOKrt 2.8KRT8MGC118110MGC174782MGC53564MGC85764sb:cb186Type-II keratin Kb8
Calculated MW
54 kDa
Observed MW
54 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:200-1:800
Concentration
430 μg/mL
Storage Buffer
PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas