Cleaved-CASP3 p12 (D175) Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD230003A
Western Blot analysis of KB cells using Cleaved-CASP3 p12 (D175) Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of Human liver cancer tissue using Cleaved-CASP3 p12 (D175) Polyclonal Antibody at dilution of 1:200.
Product Name
Cleaved-CASP3 p12 (D175) Polyclonal Antibody
Catalog Number
RD230003A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
Immunogen Information
Immunogen
Synthesized peptide derived from the Internal region of human Caspase-3 p12
Swissprot
P42574
Synonyms
A830040C14RikApopainCASP-3CASP3CASP3Casp3aCaspase 3Caspase 3apoptosis-related cysteine peptidaseCaspase 3apoptosis-related cysteine proteaseCaspase 3apoptosis-related cysteine protease aCaspase-3 subunit p12CC3CPP-32CPP32CPP32BCysteine p
Calculated MW
32 kDa
Observed MW
20+35 kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-2000, IHC 1:50-300, IF 1:50-300
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas