Cleaved PARP (Asp214) Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90365A
Western blot analysis of Jurkat using Cleaved PARP Polyclonal Antibody at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Product Name
Cleaved PARP (Asp214) Polyclonal Antibody
Catalog Number
RD90365A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Immunogen Information
Immunogen
Recombinant fusion protein of human Cleaved PARP
Gene ID
142
Swissprot
P09874
Synonyms
ADPRTADPRT 1ADPRT1ARTD1PARPPARP-1PPOLpADPRT-1PARP1Cleaved PARPPARP1
Calculated MW
252kDa
Observed MW
270-300KDa
Applications
Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.