Cleaved-PARP1 (D214) Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD70698A
Western Blot analysis of A549 cells using Cleaved-PARP1 (D214) Polyclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Human lung cancer tissue using Cleaved-PARP1 (D214) Polyclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Rat spleen tissue using Cleaved-PARP1 (D214) Polyclonal Antibody at dilution of 1:200.
Product Name
Cleaved-PARP1 (D214) Polyclonal Antibody
Catalog Number
RD70698A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Immunogen Information
Immunogen
Synthesized peptide derived from the Internal region of human PARP-1
Swissprot
P09874
Synonyms
ADP-ribosyltransferase diphtheria toxin-like 1ADPRT 1ADPRTADPRT1APOPAINARTD1NAD(+) ADP-ribosyltransferase 1PARPPARP-1PARP1PARP1Poly [ADP-ribose] polymerase 1Poly ADP ribose polymerase 1Poly[ADP-ribose] synthase 1PPOLSCA1
Calculated MW
113 kDa
Observed MW
24 kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-2000, IHC 1:50-300, IF 1:50-300
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.