For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86869A
Western blot analysis of NIH/3T3 using COX1 Polyclonal Antibody at 1:400 dilution.
Immunohistochemistry of paraffin-embedded human liver using COX1 Polyclonal Antibody at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Enables cytochrome-c oxidase activity. Predicted to be involved in electron transport coupled proton transport; mitochondrial electron transport, cytochrome c to oxygen; and response to oxidative stress. Located in mitochondrial inner membrane. Part of mitochondrial respiratory chain complex IV. Is expressed in several structures, including brown fat; heart; liver; metanephros; and skeletal muscle. Orthologous to human MT-CO1 (mitochondrially encoded cytochrome c oxidase I).
Immunogen Information
Immunogen
A synthetic peptide of mouse COX1
Gene ID
17708
Swissprot
P22437
Synonyms
MT-CO1COIMTCO1COX1
Calculated MW
223kDa
Observed MW
250KDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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