Cox2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86760A
Western blot analysis of various lysates using COX2/PTGS2 Polyclonal Antibody at 1:500 dilution.
Western blot analysis of Mouse lung using COX2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human breast cancer using COX2/PTGS2 Polyclonal antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human colon using COX2/PTGS2 Polyclonal antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Variation
Quantity
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Overview
Product Name
Cox2 Polyclonal Antibody
Catalog Number
RD86760A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
Immunogen Information
Immunogen
Recombinant fusion protein of human Cox2
Gene ID
5743
Swissprot
P35354
Synonyms
PTGS2COX-2COX2GRIPGHSPGG/HSPGHS-2PHS-2hCox-2Cox2
Calculated MW
46kDa
Observed MW
47kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:1000-1:4000,IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas