CRACR2A Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot analysis of extracts of Mouse liver using CRACR2A Polyclonal Antibody at dilution of 1:3000.
Immunohistochemistry of paraffin-embedded Human colon using CRACR2A Polyclonal Antibody at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded Human stomach using CRACR2A Polyclonal Antibody at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded Human appendix using CRACR2A Polyclonal Antibody at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse spleen using CRACR2A Polyclonal Antibody at dilution of 1:150 (40x lens).
Product Name
CRACR2A Polyclonal Antibody
Catalog Number
RD81616A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Ca2+-binding protein that plays a key role in store-operated Ca2+entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca2+concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca2+resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex.
Immunogen Information
Immunogen
Recombinant fusion protein of human CRACR2A (NP_001138430.1).
Gene ID
84766
Swissprot
Q9BSW2
Synonyms
CRACR2AEFCAB4B
Calculated MW
45 kDa/83 kDa
Observed MW
45 kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas