For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86890A
Western blot analysis of extracts of various cell lines using NY-ESO-1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human testis using NY-ESO-1 Polyclonal Antibody at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.
The protein encoded by this gene is an antigen that is overexpressed in many cancers but that is also expressed in normal testis. This gene is found in a duplicated region of the X-chromosome and therefore has a neighboring gene of identical sequence.
Immunogen Information
Immunogen
Recombinant fusion protein of human CTAG1B
Gene ID
1485
Swissprot
P78358
Synonyms
CTAG1BCT6.1CTAGCTAG1ESO1LAGE-2LAGE2BNY-ESO-1
Calculated MW
42kDa/113kDa
Observed MW
150kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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