For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90071A
Western blot analysis of extracts of U2OS cells using CTGF Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Mouse spinal cord using CTGF Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat kidney using CTGF Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human colon using CTGF Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 using CTGF Polyclonal Antibody at dilution of 1 : 150 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HepG2 using CTGF Polyclonal Antibody at dilution of 1 : 150 (40x lens). Blue: DAPI for nuclear staining.
The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.
Immunogen Information
Immunogen
A synthetic peptide of human CTGF
Gene ID
1490
Swissprot
P29279
Synonyms
CTGFCCN2HCS24IGFBP8NOV2
Calculated MW
69kDa
Observed MW
60KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.