CUL4A Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD219279A
Western blot analysis of 231 and HepG2 cell lysates using CUL4A Polyclonal Antibody at dilution of 1:600
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CUL4A Polyclonal Antibody at dilution of 1:55(×200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CUL4A Polyclonal Antibody at dilution of 1:55(×200)
Product Name
CUL4A Polyclonal Antibody
Catalog Number
RD219279A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
Cullin proteins assemble a large number of RING E3 ubiquitin ligases,participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins,CUL4A (87 kDa) and CUL4B(104 kDa),have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases,DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol,suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.
Immunogen Information
Immunogen
Synthetic peptide of human CUL4A
Swissprot
Q13619
Synonyms
2810470J21RikAW495282CUL 4ACUL-4ACul4aCul4a proteinCUL4ACullin-4AMGC36573MGC64071
Calculated MW
88 kDa
Observed MW
Refer to figures
Gene Accession
NP001008895
Applications
Reactivity
Human, Mouse
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:50-1:300, ELISA 1:5000-1:10000
Concentration
1.62 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas