For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88818A
Western blot analysis of extracts of various cell lines using CXCL4/PF4 Polyclonal Antibody at 1:500 dilution.
Immunohistochemistry of paraffin-embedded rat bone marrow using CXCL4/PF4 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of rat bone marrow using CXCL4/PF4 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. The protein also exhibits antimicrobial activity against Plasmodium falciparum.
Immunogen Information
Immunogen
Recombinant fusion protein of human CXCL4/PF4
Gene ID
5196
Swissprot
P02776
Synonyms
PF-4CXCL4SCYB4PF4
Calculated MW
103kDa
Observed MW
100kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.