CYB5A Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD83278A
Western blot analysis of extracts of various cell lines using CYB5A Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat kidney using CYB5A Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse heart using CYB5A Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using CYB5A Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
CYB5A Polyclonal Antibody
Catalog Number
RD83278A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human CYB5A (NP_683725.1).
Gene ID
1528
Swissprot
P00167
Synonyms
CYB5ACYB5MCB5
Calculated MW
11 kDa/14 kDa/15 kDa
Observed MW
17 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas