Cyclin B1 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD70323A
Western Blot analysis of various samples using Cyclin B1 Polyclonal Antibody at dilution of 1:500.
Western Blot analysis of various samples using Cyclin B1 Polyclonal Antibody at dilution of 1:500.
Immunohistochemistry analysis of paraffin-embedded Human tonsil  using Cyclin B1 Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Cyclin B1 Polyclonal Antibody at dilution of 1:300.
Immunofluorescence analysis of paraffin-embedded Human lung cancer  using Cyclin B1 Polyclonal Antibody at dilution of 1:100.
Immunofluorescence analysis of paraffin-embedded Human lung cancer using Cyclin B1 Polyclonal Antibody at dilution of 1:100.
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Overview

Circle
Product Name
Cyclin B1 Polyclonal Antibody
Catalog Number
RD70323A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
G2/mitotic-specific cyclin-B1 is a protein that in humans is encoded by the CCNB1 gene. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Immunogen Information

Circle
Immunogen
KLH conjugated Synthetic peptide corresponding to Mouse Cyclin B1
Swissprot
P14635,P24860,P30277
Synonyms
CCNB 1CCNBccnb1CCNB1Cyclin B1G2 mitotic specific cyclin B1G2/mitotic-specific cyclin-B1
Calculated MW
55 kDa
Observed MW
55 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:300-1:500, IF 1:100-1:300
Concentration
270 μg/mL
Storage Buffer
PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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