CYCS Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD70247A
Western Blot analysis of various samples using CYCS Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using CYCS Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of paraffin-embedded mouse kidney using CYCS Polyclonal Antibody at dilution of 1:500.
Immunofluorescence analysis of paraffin-embedded mouse liver using CYCS Polyclonal Antibody at dilution of 1:500.
Immunohistochemistry analysis of paraffin-embedded Human Heart using CYCS Polyclonal Antibody.
Product Name
CYCS Polyclonal Antibody
Catalog Number
RD70247A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation,cytochrome c can be released from mitochondria into cytoplasm,resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition,cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.
Immunogen Information
Immunogen
KLH conjugated Synthetic peptide corresponding to Mouse Cytochrome c
Swissprot
P99999,P62898,P62897
Synonyms
Cytochrome CSomaticCYCCytochrome CTHC4HCS.
Calculated MW
12 kDa
Observed MW
12 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:500-1:1000, IF 1:200-1:800
Concentration
1.5 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.