Cyt-C Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90754A
Western blot with Cyt-C Polyclonal antibody at dilution of 1:500.lane 1:HepG2 whole cell lysate,lane 2:MCF-7 whole cell lysate,lane 3:Jurkat whole cell lysate,lane 4:Molt-4 whole cell lysate,lane 5:C2C12 whole cell lysate,lane 6:Mouse brain
Product Name
Cyt-C Polyclonal Antibody
Catalog Number
RD90754A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain,cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides,cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation,cytochrome c can be released from mitochondria into cytoplasm,which is required for caspase-3 activation and the occurrence of apoptosis.
Immunogen Information
Immunogen
Recombinant Human Cyt-C protein expressed by? E.coli
Swissprot
P99999
Synonyms
CYCCYCScyto cCytochrome ccytochrome csomaticHCSTHC4
Calculated MW
55kDa
Observed MW
160kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:250-1:500
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.