Cytochrome c Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86773A
Western blot analysis of extracts of various cell lines using Cytochrome C Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat lung using Cytochrome C Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human tonsil using Cytochrome C Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver cancer using Cytochrome C Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using Cytochrome C Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of HepG2 cells using Cytochrome C Polyclonal antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Cytochrome C Polyclonal antibodyat dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
Cytochrome c Polyclonal Antibody
Catalog Number
RD86773A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.
Immunogen Information
Immunogen
Recombinant fusion protein of human Cytochrome c
Gene ID
54205
Swissprot
P99999
Synonyms
CYCSCYCHCSTHC4cytochrome c
Calculated MW
69kDa/79kDa
Observed MW
79kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.