DBT Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89268A
Western blot analysis of extracts of various cell lines using DBT Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse kidney using DBT Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A-431 cells using DBT Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using DBT Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
DBT Polyclonal Antibody
Catalog Number
RD89268A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Immunogen Information
Immunogen
Recombinant fusion protein of human DBT
Gene ID
1629
Swissprot
P11182
Synonyms
E2E2BBCATE2BCKADE2BCKAD-E2BCKDH-E2BCOADC-E2
Calculated MW
24kDa
Observed MW
35KDa/75KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.