DDC Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89582A
Western blot analysis of extracts of various cell lines using DDC Polyclonal Antibody at 1:500 dilution.
Immunohistochemistry of paraffin-embedded human small intestine using DDC Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using DDC Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse stomach using DDC Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat brain using DDC Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat kidney using DDC Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of mouse kidney cells using DDC Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
DDC Polyclonal Antibody
Catalog Number
RD89582A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human DDC
Gene ID
1644
Swissprot
P20711
Synonyms
DDCAADC
Calculated MW
33kDa
Observed MW
36kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:200-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas