DDIT3/CHOP Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90293A
Western blot analysis of extracts of various cell lines using DDIT3/CHOP Polyclonal Antibody at 1:1000 dilution.C2C12 and C6 cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Immunohistochemistry of paraffin-embedded Human esophageal cancer using VDR Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver cancer using VDR Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Name
DDIT3/CHOP Polyclonal Antibody
Catalog Number
RD90293A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
Immunogen Information
Immunogen
Recombinant fusion protein of human DDIT3/CHOP
Gene ID
1649
Swissprot
P35638
Synonyms
DDIT3CEBPZCHOPCHOP-10CHOP10GADD153DNA damage inducible transcript 3
Calculated MW
144kDa/183kDa/184kDa
Observed MW
Refer to figures
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.