DDX31 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD266617A
Western blot analysis of extracts of A-549 cells using DDX31 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of A-549 cells using DDX31 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of HeLa cells using DDX31 Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using DDX31 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using DDX31 Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using DDX31 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
DDX31 Polyclonal Antibody
Catalog Number
RD266617A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human DDX31 (NP_073616.6).
Gene ID
64794
Swissprot
Q9H8H2
Synonyms
DDX31PPP1R25
Calculated MW
34 kDa/64 kDa/85 kDa/94 kDa
Observed MW
94 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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